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IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08416

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # IGF1/2872R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human IGF-I/IGF-1 protein (Uniprot: P05019)

Localization

Cytoplasmic (Secreted)

Specificity

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07381).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free

Flow Cytometry: IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free [NBP3-08416]

Flow Cytometry: IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free [NBP3-08416]

Flow Cytometry: IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free [NBP3-08416] - Flow Cytometric Analysis of human K562 cells using IGF-I/IGF-1 Rabbit Recombinant Monoclonal Antibody (IGF1/2872R) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype control (Red).

Applications for IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IGF-I/IGF-1

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IGF-I/IGF-1 Antibody (IGF1/2872R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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