IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16685
![Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] AntibodyAzide and BSA Free [NBP3-16685]](https://resources.bio-techne.com/images/products/IGF1R-Insulin-R-%5Bp-Tyr1190-p-Tyr1189-p-Tyr1166-p-Tyr1165%5D-Antibody-Western-Blot-NBP3-16685-img0001.jpg "Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] AntibodyAzide and BSA Free [NBP3-16685]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary
Immunogen
A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1R/Insulin R (NP_000199.2). YETDYY
Modification
p Tyr 1165, p Tyr 1166, p Tyr 1189, p Tyr 1190
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
![Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] AntibodyAzide and BSA Free [NBP3-16685]](https://resources.bio-techne.com/images/products/IGF1R-Insulin-R-[p-Tyr1190-p-Tyr1189-p-Tyr1166-p-Tyr1165]-Antibody-Western-Blot-NBP3-16685-img0001.jpg "Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] AntibodyAzide and BSA Free [NBP3-16685]")
Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] AntibodyAzide and BSA Free [NBP3-16685]
Western Blot: IGF1R/Insulin R [p Tyr1190, p Tyr1189, p Tyr1166, p Tyr1165] Antibody [NBP3-16685] - Western blot analysis of extracts of 293T cells, using IGF1R/Insulin R antibody (NBP3-16685) at 1:1000 dilution or IGF1R antibody.293T cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.Applications
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Application Notes
Modifications: IGF1R - p Tyr 1165/1166, Insulin R - p Tyr 1189/1190
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IGF1R/Insulin R
Alternate Names
CD220, CD221, HHF5, IGF-I receptor, INSR, insulin receptor, insulin-like growth factor 1 receptor, IR, JTK13
Gene Symbol
IGF1R
Additional IGF1R/Insulin R Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov