IGF2BP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52044

Novus Biologicals, part of Bio-Techne
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NBP1-52044
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IGF2BP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKVFAEHKISYSGQ corresponding to internal region according to NP_001153895.1.

Epitope

C-EKVFAEHKISYSGQ

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Specificity

This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IGF2BP1 Antibody

Western Blot: IGF2BP1 Antibody [NBP1-52044]

Western Blot: IGF2BP1 Antibody [NBP1-52044]

Western Blot: IGF2BP1 Antibody [NBP1-52044] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-52044]

Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-52044]

Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-52044] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for IGF2BP1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-1ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1:100-1:2000
Application Notes
Western blot: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3). Primary incubation was 1 hour
IHC: Paraffin embedded Human Breast.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGF2BP1

The IGF2BP1 gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including

Alternate Names

Coding region determinant-binding protein, CRD-BP, CRDBPIGF-II mRNA-binding protein 1, IGF II mRNA binding protein 1, IMP1, IMP-1ZBP-1, insulin-like growth factor 2 mRNA binding protein 1, insulin-like growth factor 2 mRNA-binding protein 1, VICKZ family member 1, VICKZ1, ZBP1IGF2 mRNA-binding protein 1, Zip code-binding protein 1, Zipcode-binding protein 1

Entrez Gene IDs

10642 (Human); 140486 (Mouse); 303477 (Rat)

Gene Symbol

IGF2BP1

UniProt

NP_006537.3 (Human)

Additional IGF2BP1 Products

Product Documents for IGF2BP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IGF2BP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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