IGF2BP3 Antibody (JE34-04)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32471
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE34-04
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human IGF2BP3 aa 1-250 / 579. (Uniprot: O00425)
Localization
Nucleus, Cytoplasm, P-body, Stress granule.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IGF2BP3 Antibody (JE34-04)
Western Blot: IGF2BP3 Antibody (JE34-04) [NBP3-32471]
Western Blot: IGF2BP3 Antibody (JE34-04) [NBP3-32471] - Western blot analysis of IGF2BP3 on different lysates with Rabbit anti-IGF2BP3 antibody (NBP3-32471) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: K-562 cell lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 64 kDa
Observed band size: 70 kDa
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32471) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: IGF2BP3 Antibody (JE34-04) [NBP3-32471]
Immunohistochemistry: IGF2BP3 Antibody (JE34-04) [NBP3-32471] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-IGF2BP3 antibody (NBP3-32471) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32471) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: IGF2BP3 Antibody (JE34-04) [NBP3-32471]
Immunocytochemistry/ Immunofluorescence: IGF2BP3 Antibody (JE34-04) [NBP3-32471] - Immunocytochemistry analysis of HeLa cells labeling IGF2BP3 with Rabbit anti-IGF2BP3 antibody (NBP3-32471) at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF2BP3 antibody (NBP3-32471) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Applications for IGF2BP3 Antibody (JE34-04)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGF2BP3
Alternate Names
CT98, hKOC, IGF II mRNA binding protein 3, IGF2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IMP-3KH domain-containing protein overexpressed in cancer, IMP3VICKZ family member 3, insulin-like growth factor 2 mRNA binding protein 3, insulin-like growth factor 2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, KOC1DKFZp686F1078, VICKZ3cancer/testis antigen 98
Gene Symbol
IGF2BP3
Additional IGF2BP3 Products
Product Documents for IGF2BP3 Antibody (JE34-04)
Product Specific Notices for IGF2BP3 Antibody (JE34-04)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...