IGFBP-2 Antibody (4B2G6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16733

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16733-100ul
NBP3-16733-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B2G6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IGFBP-2 Antibodies »

Product Summary for IGFBP-2 Antibody (4B2G6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 226-325 of human IGFBP-2 (P18065). PCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGFBP-2 Antibody (4B2G6)

Western Blot: IGFBP-2 Antibody (4B2G6) [NBP3-16733]

Western Blot: IGFBP-2 Antibody (4B2G6) [NBP3-16733]

Western Blot: IGFBP-2 Antibody (4B2G6) [NBP3-16733] - Western blot analysis of extracts of various cell lines, using IGFBP-2 antibody (NBP3-16733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: IGFBP-2 Antibody (4B2G6) [NBP3-16733]

Immunohistochemistry-Paraffin: IGFBP-2 Antibody (4B2G6) [NBP3-16733]

Immunohistochemistry-Paraffin: IGFBP-2 Antibody (4B2G6) [NBP3-16733] - Immunohistochemistry of paraffin-embedded human colon using IGFBP-2 Rabbit mAb (NBP3-16733) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for IGFBP-2 Antibody (4B2G6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGFBP-2

IGFBP2 is a member of the ISGBP family which bind various IGFs. IGFBP2 is over expressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The over expression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.

Long Name

Insulin-like Growth Factor Binding Protein 2

Alternate Names

IGFBP2

Gene Symbol

IGFBP2

Additional IGFBP-2 Products

Product Documents for IGFBP-2 Antibody (4B2G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IGFBP-2 Antibody (4B2G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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