IGFBP-rp1/IGFBP-7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-94904

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-94904
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IGFBP-rp1/IGFBP-7 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NKVKRGHYGVQRTE corresponding to internal region according to NP_001544.1 .

Epitope

C-NKVKRGHYGVQRTE

Reactivity Notes

Expected from sequence similarity: Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IGFBP-rp1/IGFBP-7 Antibody

Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904]

Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904]

Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904] - (2.5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904]

Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904]

Immunohistochemistry-Paraffin: IGFBP-rp1/IGFBP-7 Antibody [NB100-94904] - (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for IGFBP-rp1/IGFBP-7 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
Western blot: Preliminary experiments gave an approx 50kDa band in Human Kidney, Spleen, Ovary, Prostate and Testis lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.1kDa according to NP_001544.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Tonsil and Pancreas.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGFBP-rp1/IGFBP-7

Observational study of gene-disease association. (HuGE Navigator. A genome-wide RNA-interference screening to identify genes required for an activated BRAF oncogene to block proliferation of fibroblasts and melanocytes revealed that a IGFBP7, has a central role in BRAF-mediated senescence and apoptosis. This study supports the role of IGFBP-1, -3 and -7 as potential tumour suppressor genes in human breast cancer. IGFBP7 plays a potential tumor suppressor role in colorectal carcinogenesis. Expression in tumor cells may reduce the anchorage-independent growth ability, leading to the marked loss of tumorigenicity. discovered the implication of insulin-like growth factor-binding protein-related protein 1 in endometrial physiology, which seems related to endometrial receptivity. data suggest that mac25/IGFBP-rP1 and 25.1 may play a functional role in the NE differentiation of NSCLC cell lines and may provide a novel therapeutic target for treating lung cancers, in particular NSCLC with NE differentiation. findings show for the first time that circulating IGF-binding protein (IGFBP)-related protein 1 (IGFBP-rP1) is increased with insulin resistance. that IGFBP-rP1 is an inhibitor of MCF-7 breast cancer cell proliferation and may act via a cellular senescence-like mechanism. IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1. mRNA expression was lost in six out of eight CRC cell lines as compared to normal colon cells. DNA methylation was found in the region of exon 1 and intron 1 of IGFBP-7. tumor-suppressive activity is through induction of apoptosis in an IGF-I independent manner in prostate cancer. SOX9 contributes to growth regulation by mac25 via inhibition of cell growth and promotion of differentiation. In prostate cancer cells, one of the downstream mediators of the senescence-associated tumor suppression effect of mac25/IGFBP-rP1 is superoxide dismutase 2

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Alternate Names

IGFBP-7, IGFBP7, IGFBPrp1, Mac25, PSF

Entrez Gene IDs

3490 (Human)

Gene Symbol

IGFBP7

UniProt

NP_001544.1 (Human)

Additional IGFBP-rp1/IGFBP-7 Products

Product Documents for IGFBP-rp1/IGFBP-7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IGFBP-rp1/IGFBP-7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.