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IgG Antibody (RMG03) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25960

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25960

Key Product Details

Species Reactivity

Rabbit

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Goat IgG Clone # RMG03

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Rabbit IgG

Specificity

This antibody reacts to Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

Clonality

Monoclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IgG Antibody (RMG03) - Azide and BSA Free

IgG Antibody (RMG03) - Azide and BSA Free

ELISA: IgG Antibody (RMG03) - Azide and BSA Free [NBP3-25960] -

ELISA: IgG Antibody (RMG03) - Azide and BSA Free [NBP3-25960] - ELISA of IgGs from different species shows RMG03 reacts to rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG03 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
IgG Antibody (RMG03) - Azide and BSA Free

ELISA: IgG Antibody (RMG03) - Azide and BSA Free [NBP3-25960] -

ELISA: IgG Antibody (RMG03) - Azide and BSA Free [NBP3-25960] - A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of RMG03 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

Applications for IgG Antibody (RMG03) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.01 ug/ml - 0.5 ug/ml

Western Blot

0.1 ug/ml - 0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for IgG Antibody (RMG03) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IgG Antibody (RMG03) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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