Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # IG1707R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IgG Heavy Chain Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IgG Heavy Chain protein

Localization

Cytoplasm, Cell Surface and Secreted

Specificity

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of recombinant monoclonal antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53149).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free

Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free [NBP2-54449]

Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free [NBP2-54449]

Immunohistochemistry-Paraffin: IGHG1 Antibody (IG1707R) - Azide and BSA Free [NBP2-54449] - Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Rabbit Recombinant Monoclonal Antibody (IG1707R).

Applications for IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IgG Heavy Chain

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Heavy Chain Products

Product Documents for IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IgG Heavy Chain Antibody (IG1707R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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