IgG Heavy Chain Antibody (RM116) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62019B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM116
Concentration
1 mg/ml
Product Specifications
Immunogen
Human IgG
Specificity
This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma 2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG Heavy Chain Antibody (RM116) [Biotin]
Immunohistochemistry: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] - Immunohistochemistry of Human Tonsil using Anti-Gamma Heavy Chain antibody NBP2-62019B.
ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] - A titer ELISA using NBP2-62019B. The plate was coated with different amounts of human IgG1. A serial dilution of NBP2-62019B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] - ELISA of human immunoglobulins shows NBP2-62019B reacted to the gamma1, gamma2, gamma3, gamma4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of NBP2-62019B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for IgG Heavy Chain Antibody (RM116) [Biotin]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG Heavy Chain
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Heavy Chain Products
Product Documents for IgG Heavy Chain Antibody (RM116) [Biotin]
Product Specific Notices for IgG Heavy Chain Antibody (RM116) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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