IgG2a Antibody (RM219) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62008B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM219
Concentration
1 mg/ml
Product Summary for IgG2a Antibody (RM219) [Biotin]
Immunogen
Mouse IgG2a
Specificity
This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG2a Antibody (RM219) [Biotin]
Western Blot: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5 ug/mL of NBP2-62008B. This antibody reacts to nonreduced IgG2a (~150 kDa).
ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - ELISA of mouse immunoglobulins shows NBP2-62008B reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins. NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for IgG2a Antibody (RM219) [Biotin]
Application
Recommended Usage
ELISA
0.005 ug/ml - 0.2 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for IgG2a Antibody (RM219) [Biotin]
Product Specific Notices for IgG2a Antibody (RM219) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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