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IGSF2/CD101 Antibody (BB27) [PE/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65269PECY7

Clone BB27 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65269PECY7
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BB27

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Human thymic clone B12

Specificity

NB100-65269 recognizes human CD101. CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. Clone BB27 has been reported to inhibit allogeneic T-cell responses (1). Removal of sodium azide is recommended prior to use in functional assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for IGSF2/CD101 Antibody (BB27) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: IGSF2/CD101

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Gene Symbol

CD101

Additional IGSF2/CD101 Products

Product Documents for IGSF2/CD101 Antibody (BB27) [PE/Cy7]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IGSF2/CD101 Antibody (BB27) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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