IkB-beta Antibody (1G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00004793-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IkB-beta Antibodies »

Product Specifications

Immunogen

NFKBIB (AAH15528, 1 a.a. ~ 356 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

Specificity

NFKBIB - nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (1G6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IkB-beta Antibody (1G6)

Western Blot: IkB-beta Antibody (1G6) [H00004793-M06]

Western Blot: IkB-beta Antibody (1G6) [H00004793-M06]

Western Blot: IkB-beta Antibody (1G6) [H00004793-M06] - Analysis of NFKBIB expression in transfected 293T cell line by NFKBIB monoclonal antibody (M06), clone 1G6. Lane 1: NFKBIB transfected lysatE (37.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: IkB-beta Antibody (1G6) [H00004793-M06]

Immunocytochemistry/ Immunofluorescence: IkB-beta Antibody (1G6) [H00004793-M06]

Immunocytochemistry/Immunofluorescence: IkB-beta Antibody (1G6) [H00004793-M06] - Analysis of monoclonal antibody to NFKBIB on HeLa cell. Antibody concentration 40 ug/ml
Sandwich ELISA: IkB-beta Antibody (1G6) [H00004793-M06] - Detection limit for recombinant GST tagged NFKBIB is 0.1 ng/ml as a capture antibody.

Applications for IkB-beta Antibody (1G6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IkB-beta

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Long Name

I-kappa-B-beta

Alternate Names

IkBbeta, NFKBIB, TR-interacting protein 9

Entrez Gene IDs

4793 (Human)

Gene Symbol

NFKBIB

UniProt

AAH15528 (Human)

Additional IkB-beta Products

Product Documents for IkB-beta Antibody (1G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IkB-beta Antibody (1G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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