IKK alpha Antibody (14A231) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27409

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 14A231

Format

Azide Free

Concentration

1.0 mg/ml

View all IKK alpha Antibodies »

Product Specifications

Immunogen

This antibody was raised against a His-tagged full-length human IKK alpha protein.

Reactivity Notes

New World Monkey

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IKK alpha Antibody (14A231) - Azide Free

Western Blot: IKK alpha Antibody (14A231)Azide Free [NBP2-27409]

Western Blot: IKK alpha Antibody (14A231)Azide Free [NBP2-27409]

Western Blot: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Total protein from various Human, Mouse and Rat cell lines were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-IKK-alpha in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image using the BSA free format of this product.
Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunocytochemistry/ Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-IKK alpha Antibody (14A231) at 2 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409]

Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - FFPE human kidney stained with IKKa antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications for IKK alpha Antibody (14A231) - Azide Free

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 24804954)

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Frozen

reported in scientific literature (PMID 25133425)

Immunohistochemistry-Paraffin

5ug/ml

Immunoprecipitation

1-2ug/ml

Western Blot

1ug/ml
Application Notes
An 85 kDa band should be observed. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK alpha

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkB is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, Ikka, Ikk2/, and Ikk3/. In vitro, Ikka and Ikk2/ can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. Ikka and Ikk2/ are phosphorylated by NF-kB-inducing kinase (NIk) and MAP kinase kinase kinase-1 (MEkk1) (1), respectively. Targeted disruption of Ikka gene in mice results in skin and limb abnormalities and death of newborns (2,3).

Long Name

IkB Kinase alpha

Alternate Names

CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16

Entrez Gene IDs

1147 (Human); 12675 (Mouse)

Gene Symbol

CHUK

Additional IKK alpha Products

Product Documents for IKK alpha Antibody (14A231) - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK alpha Antibody (14A231) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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