IKK beta Antibody (3C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00003551-M08

Novus Biologicals, part of Bio-Techne
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H00003551-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IKK beta Antibodies »

Product Specifications

Immunogen

IKBKB (AAH06231, 3 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREG

Specificity

IKBKB (3C12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IKK beta Antibody (3C12)

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08]

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08] - Analysis of monoclonal antibody to IKBKB on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: IKK beta Antibody (3C12) [H00003551-M08] - Detection limit for recombinant GST tagged IKBKB is approximately 0.3ng/ml as a capture antibody.

Applications for IKK beta Antibody (3C12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IKK beta

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human)

Gene Symbol

IKBKB

OMIM

603258 (Human)

UniProt

AAH06231 (Human)

Additional IKK beta Products

Product Documents for IKK beta Antibody (3C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IKK beta Antibody (3C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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