IKK beta Antibody (5D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26205

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK beta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human IKK beta [UniProt O14920]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK beta Antibody (5D12)

IKK beta Antibody (5D12)

Western Blot: IKK beta Antibody (5D12) [NBP3-26205] -

Western Blot: IKK beta Antibody (5D12) [NBP3-26205] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate.
All lanes: IKK beta Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 87, 86, 30, 80 kDa
Observed band size: 87 kDa

Applications for IKK beta Antibody (5D12)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

Additional IKK beta Products

Product Documents for IKK beta Antibody (5D12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK beta Antibody (5D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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