IKK beta Antibody (8X6V3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15810

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15810-100ul
NBP3-15810-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8X6V3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK beta Antibodies »

Product Summary for IKK beta Antibody (8X6V3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 450-756 of human IKK beta (O14920). GQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK beta Antibody (8X6V3)

Western Blot: IKK beta Antibody (8X6V3) [NBP3-15810]

Western Blot: IKK beta Antibody (8X6V3) [NBP3-15810]

Western Blot: IKK beta Antibody (7G8J8) [NBP3-15810] - Western blot analysis of extracts of various cell lines, using IKK beta antibody (NBP3-15810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (8X6V3) [NBP3-15810]

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (8X6V3) [NBP3-15810]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (7G8J8) [NBP3-15810] - Immunofluorescence analysis of PC-12 cells using IKK beta Rabbit mAb (NBP3-15810) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: IKK beta Antibody (8X6V3) [NBP3-15810]

Immunohistochemistry-Paraffin: IKK beta Antibody (8X6V3) [NBP3-15810]

Immunohistochemistry-Paraffin: IKK beta Antibody (7G8J8) [NBP3-15810] - Immunohistochemistry of paraffin-embedded human lung cancer using IKK beta antibody (NBP3-15810) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for IKK beta Antibody (8X6V3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

Additional IKK beta Products

Product Documents for IKK beta Antibody (8X6V3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IKK beta Antibody (8X6V3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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