IKK beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-477

Novus Biologicals, part of Bio-Techne
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NB600-477SS
NB600-477
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IKK beta Antibodies »

Product Specifications

Immunogen

IKK beta peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: O14920)

Reactivity Notes

This product may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the IKKb subunit.

Localization

Cytoplasmic

Specificity

Anti-IKKb may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the IKKb subunit.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IKK beta Antibody

Western Blot: IKK beta Antibody [NB600-477]

Western Blot: IKK beta Antibody [NB600-477]

Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477]

Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477]

Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.
Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477]

Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477]

Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.

Applications for IKK beta Antibody

Application
Recommended Usage

ELISA

1:1000-1:5000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:200-1:1000
Application Notes
This product was tested by immunoblot and found to be reactive against IKK-b at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-IKKb is suitable for the detection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band. Anti-IKKb has been tested in IHC using human placenta tissue.

Simple Western Antibody Database for Simple Western validation: tested in HeLa-/+EGF, Jurkat/TNF; separated by charge, antibody dilution of 1:50.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB600-477 in the following applications:

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: IKK beta

NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik-B. In turn Ik-B is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b which phosphorylate Ik-B leading to its degradation and the resulting translocation of NFk B to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a , endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse); 84351 (Rat)

Gene Symbol

IKBKB

UniProt

O14920

Additional IKK beta Products

Product Documents for IKK beta Antibody

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Certificate of Analysis

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Product Specific Notices for IKK beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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