IKK epsilon/IKBKE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33052

Novus Biologicals, part of Bio-Techne
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NBP1-33052
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IKK epsilon Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IKK epsilon/IKBKE. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKK epsilon/IKBKE Antibody

Western Blot: IKK epsilon/IKBKE Antibody [NBP1-33052]

Western Blot: IKK epsilon/IKBKE Antibody [NBP1-33052]

Western Blot: IKK epsilon/IKBKE Antibody [NBP1-33052] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody [NBP1-33052]

Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody [NBP1-33052]

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody [NBP1-33052] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody [NBP1-33052]

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody [NBP1-33052]

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody [NBP1-33052] - Paraffin-embedded human endometrium. IKBKE antibody [C2C3], C-term diluted at 1:500.
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Applications for IKK epsilon/IKBKE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IKK epsilon

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa.

Long Name

IkB Kinase epsilon

Alternate Names

IKBKE, IKK-I

Entrez Gene IDs

9641 (Human)

Gene Symbol

IKBKE

UniProt

Q14164

Additional IKK epsilon Products

Product Documents for IKK epsilon/IKBKE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IKK epsilon/IKBKE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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