Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 72C627

Concentration

1.0 mg/ml

View all IKK gamma Antibodies »

Product Specifications

Immunogen

This antibody was raised against a His-tagged full-length human IKK3/IKKg protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IKK gamma Antibody (72C627)

Western Blot: IKK gamma Antibody (72C627) [NB100-56542]

Western Blot: IKK gamma Antibody (72C627) [NB100-56542]

Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.
Simple Western: IKK gamma Antibody (72C627) [NB100-56542]

Simple Western: IKK gamma Antibody (72C627) [NB100-56542]

Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for IKK gamma Antibody (72C627)

Application
Recommended Usage

Simple Western

1:200

Western Blot

2-5 ug/ml
Application Notes
A 48 kDa band is observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK gamma

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg. This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb.

Long Name

IkB Kinase gamma

Alternate Names

FIP3P, IKBKG, IKKAP1, NEMO

Entrez Gene IDs

8517 (Human); 16151 (Mouse)

Gene Symbol

IKBKG

UniProt

Q9Y6K9

Additional IKK gamma Products

Product Documents for IKK gamma Antibody (72C627)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IKK gamma Antibody (72C627)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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