Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-1 alpha/IL-1F1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8)Azide and BSA Free [NBP2-71021]

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8)Azide and BSA Free [NBP2-71021]

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)
Immunocytochemistry/ Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021]

Immunocytochemistry/ Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021]

Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-1 alpha/IL-1F1

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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