IL-1 RI Antibody (JAMA-147) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65865

Novus Biologicals, part of Bio-Techne
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NB100-65865
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Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # JAMA-147

Format

BSA Free

Concentration

1.0 mg/ml

View all IL-1 RI Antibodies »

Product Specifications

Immunogen

Extracellular domain of murine IL-1R.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Applications for IL-1 RI Antibody (JAMA-147) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunoprecipitation

1:10-1:500
Application Notes
Measured by its ability to neutralize IL-1 alpha /IL-1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.05-0.15 ug/mL in the presence of 50 pg/mL Recombinant Mouse IL-1 alpha /IL-1F1 and 1.25 ug/mL concanavalin A.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-65865 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-1 RI

IL1 binds antigen CD121a, which is a transmembrane glycoprotein and a member of the immunoglobulin superfamily expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. CD121a binds the mature forms of IL1 alpha, IL1 beta, and IL1 Ra proteins. High affinity binding and signaling by the type I IL1 receptor requires CD121a and the accessory protein, IL1 R AcP, which does not bind IL1 alone, but forms a dimer capable of binding IL1. Interleukin 1 (IL1), which has a role as a mediator in inflammation, consists of 2 separate but related proteins, IL1 alpha and IL1 beta. Cell surface receptors for the 2 forms of IL1 are identical. IL1R Type I contains a single membrane spanning segment, a large cytoplasmic region, and an extracellular, IL1 binding portion composed of 3 immunoglobulin like domains.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Entrez Gene IDs

16177 (Mouse)

Gene Symbol

IL1R1

UniProt

P13504

Additional IL-1 RI Products

Product Documents for IL-1 RI Antibody (JAMA-147) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-1 RI Antibody (JAMA-147) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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