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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Flow Cytometry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 1B1.3a

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Purified recombinant extracellular region of mouse CDw210a

Reactivity Notes

Reacts with Mouse. Does not react with Human.

Specificity

NB100-63577 recognizes mouse CDw210a, a cell surface glycoprotein of approximately 110KD, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognised by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. This clone is reported to neutralize the effects of IL-10 (1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350]

IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350]

IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350] [NB100-63577AF350] -

IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350] [NB100-63577AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-10R alpha Antibody (1B1.3a) [Alexa Fluor® 350]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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