IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08221

Novus Biologicals, part of Bio-Techne
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NBP3-08221
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Key Product Details

Species Reactivity

Validated:

Mouse, Human (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 1B1.3a

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-10 R alpha Antibodies »

Product Summary for IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

Immunogen

Purified recombinant extracellular region of Mouse IL-10R alpha (Uniprot: Q61727)

Reactivity Notes

Does not react with Human.

Localization

Cell Surface

Specificity

This monoclonal antibody recognizes Mouse Interleukin-10 receptor 1 (IL-10R1), a cell surface glycoprotein of approximately 110KDa. Its epitope is localized in the ligand-binding domain in the extracellular region of Mouse IL-10R1. This antibody blocks the binding of IL-10 to its receptor. IL-10R1 is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Binding of IL-10 to its receptor initiates JAK/STAT signaling that leads to B cell proliferation and inhibition of macrophage activation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07330).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Neutralization Studies: Order antibody without Azide
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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