IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71030

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-10 R alpha Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL10RA(NP_001549) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

Western Blot: IL-10R alpha Antibody (OTI1D10)Azide and BSA Free [NBP2-71030]

Western Blot: IL-10R alpha Antibody (OTI1D10)Azide and BSA Free [NBP2-71030]

Western Blot: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-10 R alpha.
Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030]

Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030]

Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030]

Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030]

Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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