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IL-10R alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16941

Novus Biologicals, part of Bio-Techne
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NBP2-16941

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL-10R alpha. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-10R alpha Antibody

Western Blot: IL-10R alpha Antibody [NBP2-16941]

Western Blot: IL-10R alpha Antibody [NBP2-16941]

Western Blot: IL-10R alpha Antibody [NBP2-16941] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL10 Receptor alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: IL-10R alpha Antibody [NBP2-16941]

Immunohistochemistry-Paraffin: IL-10R alpha Antibody [NBP2-16941]

Immunohistochemistry-Paraffin: IL-10 R alpha Antibody [NBP2-16941] - Human colon carcinoma, using IL10 Receptor alpha antibody at 1:250 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: IL-10R alpha Antibody [NBP2-16941]

Western Blot: IL-10R alpha Antibody [NBP2-16941]

Western Blot: IL-10R alpha Antibody [NBP2-16941] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL10 Receptor alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for IL-10R alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-10 R alpha

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq]

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

UniProt

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-10R alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.