IL-13R alpha 1 Antibody (5A3W5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16207
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A3W5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for IL-13R alpha 1 Antibody (5A3W5)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL-13R alpha 1 (P78552). MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IL-13R alpha 1 Antibody (5A3W5)
Western Blot: IL-13R alpha 1 Antibody (5A3W5) [NBP3-16207]
Western Blot: IL-13R alpha 1 Antibody (5A3W5) [NBP3-16207] - Western blot analysis of extracts of various cell lines, using IL-13R alpha 1 antibody (NBP3-16207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: IL-13R alpha 1 Antibody (5A3W5) [NBP3-16207]
Immunohistochemistry-Paraffin: IL-13R alpha 1 Antibody (5A3W5) [NBP3-16207] - Immunohistochemistry of paraffin-embedded human lung cancer using IL-13R alpha 1 Rabbit mAb (NBP3-16207) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for IL-13R alpha 1 Antibody (5A3W5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IL-13 R alpha 1
Long Name
Interleukin 13 Receptor alpha 1
Alternate Names
CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1
Gene Symbol
IL13RA1
Additional IL-13 R alpha 1 Products
Product Documents for IL-13R alpha 1 Antibody (5A3W5)
Product Specific Notices for IL-13R alpha 1 Antibody (5A3W5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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