IL-16 Antibody (14.1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09026

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-09026-0.025mg
NBP3-09026-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # 14.1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-16 Antibodies »

Product Summary for IL-16 Antibody (14.1) - Azide and BSA Free

Immunogen

The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for IL-16 Antibody (14.1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF- alpha , and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-16

Interleukin-16 (IL-16, also known as lymphocyte chemoattractant factor (LCF)) is a CD8+ T cell-derived cytokine. It acts as a chemoattractant for CD4+ T-cells, monocytes and eosinophils. IL-16 has also been shown to suppress HIV-1 replication in vitro. It is expressed as a precursor molecule (pro-IL-16) in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The active form is assumed to be a proteolytic cleavage product of pro-IL-16 existing, under physiological conditions, predominantly as a noncovalently linked multimer. The sequence and structure of IL-16 is conserved across species, human and murine IL-16 show significant cross-species reactivity.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

Additional IL-16 Products

Product Documents for IL-16 Antibody (14.1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-16 Antibody (14.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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