IL-17/IL-17A Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42772

Novus Biologicals, part of Bio-Techne
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NBP1-42772
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-17/IL-17A Antibodies »

Product Specifications

Immunogen

This purified IL-17/IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL-17/IL-17A protein. (Uniprot: Q61453)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26356122).

Specificity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IL-17/IL-17A Antibody - Azide and BSA Free

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.
Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.
Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.
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Applications for IL-17/IL-17A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:5000

Western Blot

1:1000
Application Notes
This purified antibody has been tested for use in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kDa. By western blot from a reducing gel expect a band approximately 15.0 kDa in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IL-17/IL-17A

Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

UniProt

Q61453 (Rat)

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-17/IL-17A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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