IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12040

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 5H4

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-2 R beta Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing truncated murine IL-2R beta.

Specificity

This antibody is specific for the murine interleukin-2 receptor beta chain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free [NBP3-12040]

Immunocytochemistry/ Immunofluorescence: IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free [NBP3-12040]

Immunocytochemistry/Immunofluorescence: IL-2 R beta Antibody (5H4) - Chimeric [NBP3-12040] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 5H4 (NBP3-12040) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12040, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL-2 R beta

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Gene Symbol

IL2RB

Additional IL-2 R beta Products

Product Documents for IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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