Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 14k5H3

Concentration

1.0 mg/ml

View all IL-21 Antibodies »

Product Specifications

Immunogen

Full length recombinant protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-21 Antibody (14k5H3)

Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336]

Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336]

Western Blot: IL-21 Antibody (14k5H3) [NBP2-27336] - Analysis of IL-21. Full-length recombinant IL-21 protein probed with 1 ug/ml of IL-21 monoclonal antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for IL-21 Antibody (14k5H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27531854)

Immunohistochemistry

reported in scientific literature (PMID 27531854)

Western Blot

1-3ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-21

Interleukin-21, also alternatively named IL-17C, is a pro-inflammatory cytokine and member of the IL-17 family. The IL-17 family is comprised of at least six members: IL-17A-F (1, 2). Interleukin-21, along with IL-17B, appears to stimulate release of TNF and IL-1 from the monocytic THP-1 line thus appearing to have a different receptor and induction pattern than IL-17A (1, 2). As a pro-inflammatory cytokine IL-21 augments the inflammatory process in arthritis models (2, 3). Interleukin-21 is induced in epithelial cells by bacterial antigens and other inflammatory ligands such as imiquimod, exhibiting a direct linkage with innate immune receptors such as TLRs (2, 3). The IL-21 receptor complex appears to be comprised of IL-17RA or IL-17RE. Antibody to IL-21 allows further analysis of the unique regulatory activity, cellular receptors and cellular targets of IL-21 (IL-17C) versus other members of the IL-17 family such as IL-17A. The study of Interleukin-21 and its contribution to diseases caused by impaired epithelial barriers in autoimmune and inflammatory diseases such as psoriasis, asthma or inflammatory bowel disease potentiate development of new therapies directed at regulation of IL-21 or other IL-17 family members (4). The linkage of IL-21 with epithelial cells also furthers the study of the innate immune functions of epithelial cells.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

UniProt

Q9HBE4

Additional IL-21 Products

Product Documents for IL-21 Antibody (14k5H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-21 Antibody (14k5H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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