IL-21R Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76739
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor. The immunogen is located within amino acids 80 - 130 of IL-21 Receptor. Amino Acid Squence: NITDQSGNYSQECGS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-21R Antibody - BSA Free
Immunohistochemistry: IL-21R Antibody - BSA Free [NBP1-76739]
Immunohistochemistry: IL-21R Antibody [NBP1-76739] - Examples of negative, weak and high expression of IL-21R (brown) in human PDAC tissue. Black arrows: tumor cells. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: IL-21R Antibody - BSA Free [NBP1-76739]
Immunocytochemistry/Immunofluorescence: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in (A) immunofluorescence staining (left: IL-21R+ cells, right: Alexa488 goat anti-rabbit IgG only; nuclei: DAPI (blue), IL-21R: Alexa488 (green)). White bar: 20 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.Western Blot: IL-21R AntibodyBSA Free [NBP1-76739]
Western Blot: IL-21R Antibody [NBP1-76739] - IL-21R expression in human PDAC cell lines. Untreated human PDAC cell lines AsPC-1, BxPC-3, and Panc-1 were tested for IL-21R expression in Western Blot. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/8/9/1104) licensed under a CC-BY license.Applications for IL-21R Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
An approximately 60 kDa band can be detected in Western Blot. Use in ICC/IF was reported in scientific literature (PMID: 31540511).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-21R
Long Name
Interleukin 21 Receptor
Alternate Names
CD360, IL-21 R, IL21R, NILR
Gene Symbol
IL21R
UniProt
Additional IL-21R Products
Product Documents for IL-21R Antibody - BSA Free
Product Specific Notices for IL-21R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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