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IL-31RA Antibody (3A10)

Novus Biologicals, part of Bio-Techne | Catalog # H00133396-M01

Novus Biologicals, part of Bio-Techne
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H00133396-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

IL31RA (NP_620586, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIA

Reactivity Notes

Other species not tested.

Specificity

interleukin 31 receptor A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IL-31RA Antibody (3A10)

Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]

Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]

Western Blot: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of IL31RA over-expressed 293 cell line, cotransfected with IL31RA Validated Chimera RNAi ( Cat # H00133396-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IL31RA monoclonal antibody (M01), clone 3A10 (Cat # H00133396-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunohistochemistry-Paraffin: IL-31RA Antibody (3A10) [H00133396-M01]

Immunohistochemistry-Paraffin: IL-31RA Antibody (3A10) [H00133396-M01]

Immunohistochemistry-Paraffin: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of monoclonal antibody to IL31RA on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]

Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]

Western Blot: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of IL31RA expression in transfected 293T cell line by IL31RA monoclonal antibody (M01), clone 3A10.Lane 1: IL31RA transfected lysate(83 KDa).Lane 2: Non-transfected lysate.

Applications for IL-31RA Antibody (3A10)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-31RA

IL31RA is related to gp130 (IL6ST; MIM 600694), the common receptor subunit for IL6 (MIM 147620)-type cytokines. Oncostatin M receptor (OSMR; MIM 601743) and IL31RA form the heterodimeric receptor through which IL31 (MIM 609509) signals. Expression of IL31RA and OSMR mRNA is induced in activated monocytes, and both mRNAs are constitutively expressed in epithelial cells (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM]

Long Name

Interleukin 31 Receptor A

Alternate Names

CRL3, GLM-R, IL-31 RA, IL31RA

Entrez Gene IDs

133396 (Human)

Gene Symbol

IL31RA

OMIM

609510 (Human)

UniProt

Additional IL-31RA Products

Product Documents for IL-31RA Antibody (3A10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-31RA Antibody (3A10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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