IL-31RA Antibody (3A10)
Novus Biologicals, part of Bio-Techne | Catalog # H00133396-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
IL31RA (NP_620586, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIA
Reactivity Notes
Other species not tested.
Specificity
interleukin 31 receptor A
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for IL-31RA Antibody (3A10)
![Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]](https://resources.bio-techne.com/images/products/IL-31-RA-Antibody-3A10-Western-Blot-H00133396-M01-img0009.jpg "Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]")
Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]
Western Blot: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of IL31RA over-expressed 293 cell line, cotransfected with IL31RA Validated Chimera RNAi ( Cat # H00133396-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IL31RA monoclonal antibody (M01), clone 3A10 (Cat # H00133396-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Immunohistochemistry-Paraffin: IL-31RA Antibody (3A10) [H00133396-M01]](https://resources.bio-techne.com/images/products/IL-31-RA-Antibody-3A10-Immunohistochemistry-Paraffin-H00133396-M01-img0008.jpg "Immunohistochemistry-Paraffin: IL-31RA Antibody (3A10) [H00133396-M01]")
Immunohistochemistry-Paraffin: IL-31RA Antibody (3A10) [H00133396-M01]
Immunohistochemistry-Paraffin: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of monoclonal antibody to IL31RA on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.![Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]](https://resources.bio-techne.com/images/products/IL-31-RA-Antibody-3A10-Western-Blot-H00133396-M01-img0010.jpg "Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]")
Western Blot: IL-31RA Antibody (3A10) [H00133396-M01]
Western Blot: IL-31 RA Antibody (3A10) [H00133396-M01] - Analysis of IL31RA expression in transfected 293T cell line by IL31RA monoclonal antibody (M01), clone 3A10.Lane 1: IL31RA transfected lysate(83 KDa).Lane 2: Non-transfected lysate.Applications for IL-31RA Antibody (3A10)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: IL-31RA
Long Name
Interleukin 31 Receptor A
Alternate Names
CRL3, GLM-R, IL-31 RA, IL31RA
Entrez Gene IDs
133396 (Human)
Gene Symbol
IL31RA
OMIM
609510 (Human)
UniProt
Additional IL-31RA Products
Product Documents for IL-31RA Antibody (3A10)
Product Specific Notices for IL-31RA Antibody (3A10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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