IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71834

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-36 alpha/IL-1F6 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4)Azide and BSA Free [NBP2-71834]

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4)Azide and BSA Free [NBP2-71834]

Western Blot: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.
Immunocytochemistry/ Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834]

Immunocytochemistry/ Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834]

Immunocytochemistry/Immunofluorescence: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.
CyTOF-ready: IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71834] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IL1F6 antibody, and then analyzed by flow cytometry.

Applications for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-36 alpha/IL-1F6

Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Gene Symbol

IL36A

Additional IL-36 alpha/IL-1F6 Products

Product Documents for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IL-36 alpha/IL-1F6 Antibody (OTI1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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