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IL-5 Antibody (mepolizumab) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28010

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # mepolizumab

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

IL-5

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'mepolizumab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IL-5 Antibody (mepolizumab) - Humanized

IL-5 Antibody (mepolizumab) - Humanized

ELISA: IL-5 Antibody (mepolizumab) - Humanized[NBP3-28010] -

Immobilized human IL 5 His at 2 ug/mL can bind IL-5 Antibody (mepolizumab) - Humanized, EC50=0.1188 ug/mL.
IL-5 Antibody (mepolizumab) - Humanized

Functional: IL-5 Antibody (mepolizumab) - Humanized[NBP3-28010] -

Mepolizumab induced proliferation inhibition activity was evaluated using Human IL-5R alpha TF-1 . The EC50 was approximately 0.3282 ug/mL.
IL-5 Antibody (mepolizumab) - Humanized

Flow Cytometry: IL-5 Antibody (mepolizumab) - Humanized[NBP3-28010] -

IL-5 Antibody (mepolizumab) - Humanized FACS Blocking was evaluated using Human IL-5R beta-FL/HIL-5R alpha CHOS. The IC50 was approximately 2.844 ug/mL.

Applications for IL-5 Antibody (mepolizumab) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IL-5

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

UniProt

Additional IL-5 Products

Product Documents for IL-5 Antibody (mepolizumab) - Humanized

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-5 Antibody (mepolizumab) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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