IL-6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16957
![Western Blot: IL-6 Antibody [NBP2-16957]](https://resources.bio-techne.com/images/products/IL-6-Antibody-Western-Blot-NBP2-16957-img0006.jpg "Western Blot: IL-6 Antibody [NBP2-16957]")
![Western Blot: IL-6 Antibody [NBP2-16957]](https://resources.bio-techne.com/images/products/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "Western Blot: IL-6 Antibody [NBP2-16957]")
![Western Blot: IL-6 Antibody [NBP2-16957]](https://resources.bio-techne.com/images/products/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg "Western Blot: IL-6 Antibody [NBP2-16957]")
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Predicted:
Rhesus Macaque (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length human IL-6 Recombinant protein.
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-6 Antibody
![Western Blot: IL-6 Antibody [NBP2-16957]](https://resources.bio-techne.com/images/products/IL-6-Antibody-Western-Blot-NBP2-16957-img0016.jpg "Western Blot: IL-6 Antibody [NBP2-16957]")
Western Blot: IL-6 Antibody [NBP2-16957]
Western Blot: IL-6 Antibody [NBP2-16957] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: IL-6 Antibody [NBP2-16957]](https://resources.bio-techne.com/images/products/IL-6-Antibody-Western-Blot-NBP2-16957-img0014.jpg "Western Blot: IL-6 Antibody [NBP2-16957]")
Applications for IL-6 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NBP2-16957 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: IL-6
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Specific Notices for IL-6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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