Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 73N8F2

Concentration

1.0 mg/ml

View all IL-7R alpha/CD127 Antibodies »

Product Specifications

Immunogen

Amino acids 145-235 of mouse IL-7R / CD127 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for IL-7R alpha/CD127 Antibody (73N8F2)

Western Blot: IL-7R alpha/CD127 Antibody (73N8F2) [NBP2-25249]

Western Blot: IL-7R alpha/CD127 Antibody (73N8F2) [NBP2-25249]

Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: IL-7R alpha/CD127 Antibody (73N8F2) [NBP2-25249]

Immunohistochemistry-Paraffin: IL-7R alpha/CD127 Antibody (73N8F2) [NBP2-25249]

Immunohistochemistry-Paraffin: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Mouse testis stained with IL-7R antibody at 5 ug/ml.

Applications for IL-7R alpha/CD127 Antibody (73N8F2)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-7R alpha/CD127

IL-7R, or CD127, is often used to characterize regulatory T cells or Tregs. By phenotyping, IL-7R is inversely correlated with the high levels of the hallmark Treg transcription factor FoxP3. So lowered expression of IL-7R on the cell surface along with CD25 and CD4 in addition to FoxP3 internal expression is a major and routinely used technique for Treg cell studies. IL-7R residing on the surface enables enrichment and purification of Treg populations. Antibody to IL-7R is useful for phenotyping, identification, enumeration and purification of Tregs (1-3).

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Gene Symbol

IL7R

UniProt

NP_032398

Additional IL-7R alpha/CD127 Products

Product Documents for IL-7R alpha/CD127 Antibody (73N8F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-7R alpha/CD127 Antibody (73N8F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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