ILF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1285

Novus Biologicals, part of Bio-Techne
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NB100-1285
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ILF1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TPPAAVREKGVQN corresponding to C-Terminus according to NP_004505.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILF1 Antibody

Western Blot: ILF1 Antibody [NB100-1285]

Western Blot: ILF1 Antibody [NB100-1285]

Western Blot: ILF1 Antibody [NB100-1285] - (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NB100-1285]

Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NB100-1285]

Immunocytochemistry/Immunofluorescence: ILF1 Antibody [NB100-1285] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: ILF1 Antibody [NB100-1285]

Flow Cytometry: ILF1 Antibody [NB100-1285]

Flow Cytometry: ILF1 Antibody [NB100-1285] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for ILF1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in human thymus lysate (calculated MW of 69.1 kDa band according to NP_004505.2. Approx 80kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004505.2.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILF1

The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

Cellular transcription factor ILF-1, forkhead box K2, forkhead box protein K2, FOXK1, ILF-1, ILF1ILF, interleukin enhancer binding factor 1, Interleukin enhancer-binding factor 1

Entrez Gene IDs

3607 (Human)

Gene Symbol

FOXK2

UniProt

Q01167 (Human)

Additional ILF1 Products

Product Documents for ILF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ILF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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