ILT5/CD85a/LILRB3 Antibody (4F9) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64329PECY55
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 4F9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Monocyte derived dendritic cells
Specificity
NB100-64329 is specific for human CD85a (leucocyte immunoglobulin-like receptor, subfamily B, member 3/ LILRB3), also known as ILT-5, expressed by monocytes, dendritic cells, macrophages, subpopulation of T lymphocytes, B cells and at high levels on plasma cells. CD85a is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signalling modulators and inhibitors of cell activation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for ILT5/CD85a/LILRB3 Antibody (4F9) [PE/Cy5.5]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: LILRB3/CD85a/ILT5
Alternate Names
CD85a, ILT5, LIR3
Gene Symbol
LILRB3
Additional LILRB3/CD85a/ILT5 Products
Product Documents for ILT5/CD85a/LILRB3 Antibody (4F9) [PE/Cy5.5]
Product Specific Notices for ILT5/CD85a/LILRB3 Antibody (4F9) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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