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IMP2/IGF2BP2 Antibody (2O3W8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16585

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16585-100ul
NBP3-16585-20ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2O3W8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for IMP2/IGF2BP2 Antibody (2O3W8)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-595 of human IMP2/IGF2BP2 (NP_001007226.1). NFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVAS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IMP2/IGF2BP2 Antibody (2O3W8)

Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]

Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]

Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] - Western blot analysis of extracts of various cell lines, using IGF2BP2/IMP2/IGF2BP2 Rabbit mAb (NBP3-16585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]

Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]

Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] - Immunohistochemistry of paraffin-embedded human colon using IGF2BP2/IMP2/IGF2BP2 Rabbit mAb (NBP3-16585) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for IMP2/IGF2BP2 Antibody (2O3W8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IMP2

IGF2BP2 encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Insulin-like Growth Factor 2 mRNA Binding Protein 2

Alternate Names

IF2B2, IGF2BP2, VICKZ2

Gene Symbol

IGF2BP2

Additional IMP2 Products

Product Documents for IMP2/IGF2BP2 Antibody (2O3W8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IMP2/IGF2BP2 Antibody (2O3W8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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