Importin-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56499

Novus Biologicals, part of Bio-Techne
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NB100-56499
NB100-56499SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate, Rhesus Macaque

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Importin-9 Antibodies »

Product Summary for Importin-9 Antibody

Immunogen

A portion of amino acids 850-950 of human Importin-9 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, _b, _c), mouse (isoforms 1 and 2), 88% homologous in chicken, 83% homologous in opossum, and 78% in bovine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Importin-9 Antibody

Knockout Validated: Importin-9 Antibody [NB100-56499]

Knockout Validated: Importin-9 Antibody [NB100-56499]

Knockout Validated: Importin-9 Antibody [NB100-56499] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56499) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Importin-9 Antibody [NB100-56499]

Western Blot: Importin-9 Antibody [NB100-56499]

Western Blot: Importin-9 Antibody [NB100-56499] - Western blot analysis of Importin-9 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using NB100-56499 at 1 ug/ml.

Applications for Importin-9 Antibody

Application
Recommended Usage

Knockout Validated

1 ug/ml

Western Blot

1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Importin-9

Official Gene Symbol: IPO9 Gene ID: 55705 (human) Gene Map Locus: 1q32.1 (human) Importin-9 is a novel member of highly conserved Karyopherin Super family. Karyopherins are transport receptors consisting of an N-terminal RanGTP-binding motif and mediate the transport of protein and RNA across the nucleus and cytoplasm. These proteins interact directly with nuclear pore complex and shuttle continuously between the nucleus and cytoplasm. Importin-9 is a RAN-GTP binding protein and mediates the nuclear import of individual core histones and TBP. Reports suggest Importin-9 also interacts with subunits of PP2A (protein phosphatase 2A) and aids in nuclear import.

Alternate Names

DKFZp761M1547, FLJ10402, Imp9IMP9, importin 9, importin-9, KIAA1192, Ran-binding protein 9, RANBP9

Entrez Gene IDs

304817 (Rat)

Gene Symbol

IPO9

UniProt

Q96P70 (Human)

Additional Importin-9 Products

Product Documents for Importin-9 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Importin-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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