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Importin alpha 3/KPNA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1374

Novus Biologicals, part of Bio-Techne
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NB100-1374

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-NSSANVPTEGFQF corresponding to C-Terminus according to NP_002259.1.

Localization

Cytoplasmic and Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Importin alpha 3/KPNA4 Antibody

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-1374]

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-1374]

Western Blot: Importin alpha 3/KPNA4 Antibody [NB100-1374] - Staining of Human Ovary (A), Mouse Testes (B) with antibody at 0.1 ug/mL, Mouse Ovary (C) with antibody at 0.3 ug/mL and Rat Testes (D) lysate with antibody at 0.03 ug/mL (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.

Applications for Importin alpha 3/KPNA4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:28000

Western Blot

0.03 - 0.3 ug/mL
Application Notes
Approx 55 kDa band observed in Human and Mouse Ovary and in Mouse Testis lysates, and approx 60 kDa in Rat Testis lysates (calculated MW of 57.9 kDa according to Human NP_002259.1, Mouse NP_032493.1 and Rat NP_001014793.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin alpha 3/KPNA4

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

Long Name

Karyopherin alpha 4

Alternate Names

KPNA4, QIP12, SRP3

Entrez Gene IDs

3840 (Human)

Gene Symbol

KPNA4

Additional Importin alpha 3/KPNA4 Products

Product Documents for Importin alpha 3/KPNA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Importin alpha 3/KPNA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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