Influenza B nucleoprotein Antibody (016)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12755

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Virus

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 016

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Influenza B nucleoprotein Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Influenza B (Accession#: ACF54251.1; Met1-Tyr560).

Reactivity Notes

Pan Influenza B

Has cross-reactivity in ELISA and WB with:
Influenza B (B/Brisbane/60/2008) NP
Influenza B (B/Colorado/06/2017) NP
Influenza B (B/Florida/4/2006) NP
Influenza B (B/Phuket/3073/2013) NP
Influenza B (B/Washington/02/2019) NP

No cross-reactivity in ELISA and WB with:
H1N1 (A/Brevig Mission/1/1918) NP
H1N1 (A/Brisbane/02/2018 NP
H1N1 (A/California/07/2009) NP
H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP
H1N1 (A/Hawaii/70/2019) NP
H1N1 (A/Michigan/45/2015 NP
H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M)
H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP
H2N2 (A/Ann Arbor/6/1960) NP
H3N2 (A/Aichi/2/1968) NP
H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP
H3N2 (A/Hong Kong/1/1968) NP
H3N2 (A/Hong Kong/2671/2019) NP
H3N2 (A/Hong Kong/45/2019) NP
H3N2 (A/Hong Kong/4801/2014) NP
H3N2 (A/Kansas/14/2017) NP
H3N2 (A/Switzerland/9715293/2013) NP
H7N9 (A/Anhui/1-BALF_RG6/2013) NP
H7N9 (A/Shanghai/2/2013) NP

No cross-reactivity in ELISA with:
SARS-CoV-2 Nucleocapsid Protein
HCoV-229E Nucleocapsid Protein
HCoV-NL63 Nucleocapsid Protein
HCoV-HKU1 Nucleocapsid Protein
HCoV-OC43 Nucleocapsid Protein

Specificity

Widely recognize Influenza B Nucleoprotein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Influenza B nucleoprotein Antibody (016)

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755]

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755]

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755] - Reocombinant Protein 10 ng
Influenza B nucleoprotein Antibody (016) Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755]

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755]

NBP3-12755 used at 1:2000 dilution. Lane 1: H1N1 (A/Brevig Mission/1/1918) NP. Lane 2: H1N1 (A/Brisbane/02/2018 NP. Lane 3: H1N1 (A/California/07/2009) NP. Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP. Lane 5: H1N1 (A/Hawaii/70/2019) NP. Lane 6: H1N1 (A/Michigan/45/2015 NP. Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M). Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP. Lane 9: H2N2 (A/Ann Arbor/6/1960) NP. Lane 10: H3N2 (A/Aichi/2/1968) NP. Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP. Lane 12: H3N2 (A/Hong Kong/1/1968) NP. Lane 13: H3N2 (A/Hong Kong/2671/2019) NP. Lane 14: H3N2 (A/Hong Kong/45/2019) NP. Lane 15: H3N2 (A/Hong Kong/4801/2014 NP. Lane 16: H3N2 (A/Kansas/14/2017 NP. Lane 17: H3N2 (A/Switzerland/9715293/2013) NP. Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP. Lane 19: H7N9 (A/Shanghai/2/2013) NP. Lane 20: Influenza B (B/Brisbane/60/2008 NP. Lane 21: Influenza B (B/Colorado/06/2017 NP. Lane 22: Influenza B (B/Florida/4/2006) NP. Lane 23: Influenza B (B/Phuket/3073/2013) NP. Lane 24: Influenza B (B/Washington/02/2019) NP. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.

Applications for Influenza B nucleoprotein Antibody (016)

Application
Recommended Usage

ELISA

0.1-0.2 ug/mL

Western Blot

0.1-1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza B nucleoprotein

Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

Alternate Names

Influenza B Virus NP, NP, Nucleoprotein

Additional Influenza B nucleoprotein Products

Product Documents for Influenza B nucleoprotein Antibody (016)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Influenza B nucleoprotein Antibody (016)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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