ING5 Antibody (119)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90261

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 119

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ING5 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ING5 (Uniprot#: Q8WYH8-2; Glu36-Gln84).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ING5 Antibody (119)

Western Blot: ING5 Antibody (119) [NBP2-90261]

Western Blot: ING5 Antibody (119) [NBP2-90261]

Western Blot: ING5 Antibody (119) [NBP2-90261] - Anti-ING5 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: ING5 Antibody (119) [NBP2-90261]

Immunoprecipitation: ING5 Antibody (119) [NBP2-90261]

Immunoprecipitation: ING5 Antibody (119) [NBP2-90261] - ING5 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ING5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ING5 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 32 kDa

Applications for ING5 Antibody (119)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ING5

ING5 is encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.

Alternate Names

inhibitor of growth family, member 5, inhibitor of growth protein 5, p28ING5FLJ23842

Gene Symbol

ING5

Additional ING5 Products

Product Documents for ING5 Antibody (119)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ING5 Antibody (119)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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