Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This affinity purified ING5 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5). (Uniprot: Q8WYH8)

Reactivity Notes

Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2. However, 100% homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

Specificity

Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for ING5 Antibody

Western Blot: ING5 Antibody [NB100-1681]

Western Blot: ING5 Antibody [NB100-1681]

Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of 36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
Western Blot: ING5 Antibody [NB100-1681]

Western Blot: ING5 Antibody [NB100-1681]

Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of 45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time.

Applications for ING5 Antibody

Application
Recommended Usage

ELISA

1:4000-1:16000

Western Blot

1:500-1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa or 26 kDa in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: ING5

ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.

Alternate Names

inhibitor of growth family, member 5, inhibitor of growth protein 5, p28ING5FLJ23842

Entrez Gene IDs

84289 (Human)

Gene Symbol

ING5

UniProt

Q8WYH8 (Human)

Additional ING5 Products

Product Documents for ING5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ING5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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