Inorganic Pyrophosphatase/PPA1 Antibody (3B2)
Novus Biologicals, part of Bio-Techne | Catalog # H00005464-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3B2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPA1 (NP_066952, 10 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHT
Localization
Cytoplasmic
Specificity
PPA1 - pyrophosphatase (inorganic) 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Inorganic Pyrophosphatase/PPA1 Antibody (3B2)
![Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]](https://resources.bio-techne.com/images/products/Inorganic-Pyrophosphatase-PPA1-Antibody-3B2-Western-Blot-H00005464-M01-img0006.jpg "Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]")
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - PPA1 monoclonal antibody (M01), clone 3B2 Analysis of PPA1 expression in A-431.![Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]](https://resources.bio-techne.com/images/products/Inorganic-Pyrophosphatase-PPA1-Antibody-3B2-Western-Blot-H00005464-M01-img0005.jpg "Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]")
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01]
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - PPA1 monoclonal antibody (M01), clone 3B2. Analysis of PPA1 expression in PC-12.
Sandwich ELISA: Inorganic Pyrophosphatase/PPA1 Antibody (3B2) [H00005464-M01] - Detection limit for recombinant GST tagged PPA1 is approximately 1ng/ml as a capture antibody.
Applications for Inorganic Pyrophosphatase/PPA1 Antibody (3B2)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Inorganic Pyrophosphatase/PPA1
Alternate Names
IOPPP, IPP1, PP1, PPase, SID6-8061
Entrez Gene IDs
5464 (Human)
Gene Symbol
PPA1
OMIM
179030 (Human)
UniProt
Additional Inorganic Pyrophosphatase/PPA1 Products
Product Documents for Inorganic Pyrophosphatase/PPA1 Antibody (3B2)
Product Specific Notices for Inorganic Pyrophosphatase/PPA1 Antibody (3B2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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