Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59471

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 4G4

Format

BSA Free

Concentration

1 mg/ml

View all Inorganic Pyrophosphatase/PPA1 Antibodies »

Product Summary for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Immunogen

Recombinant human Inorganic Pyrophosphatase/PPA1 (1-289aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Scientific Data Images for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (4G4)BSA Free [NBP2-59471]

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (4G4)BSA Free [NBP2-59471]

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Lane 1: HeLa cell lysates, Lane 2: A431 cell lysates, Lane 3: 293T cell lysates, Lane 4: HepG2 cell lysates, Lane 5: U87MG cell lysates, Lane 6: PC3 cell lysates, Lane 7: WiDr cell lysates
Immunocytochemistry/ Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free [NBP2-59471]

Immunocytochemistry/ Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free [NBP2-59471]

Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Immunofluorescence analysis of Pyrophosphatase/PPA1 in HeLa cells using NBP2-59471 (1:100). The secondary antibody (green) used was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free [NBP2-59471]

Flow Cytometry: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free [NBP2-59471]

Flow Cytometry: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with NBP2-59471 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa Fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1071

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Inorganic Pyrophosphatase/PPA1

Pyrophosphatase is encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

Alternate Names

IOPPP, IPP1, PP1, PPase, SID6-8061

Gene Symbol

PPA1

Additional Inorganic Pyrophosphatase/PPA1 Products

Product Documents for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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