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Inorganic Pyrophosphatase/PPA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87787

Novus Biologicals, part of Bio-Techne
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NBP1-87787

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Inorganic Pyrophosphatase/PPA1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Inorganic Pyrophosphatase/PPA1 Antibody

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787] - Analysis using Anti-PPA1 antibody NBP1-87787 (A) shows similar pattern to independent antibody NBP1-87788 (B).
Immunocytochemistry/ Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Immunocytochemistry/ Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787]

Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-87787] - Staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.

Applications for Inorganic Pyrophosphatase/PPA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Inorganic Pyrophosphatase/PPA1

Pyrophosphatase is encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

Alternate Names

IOPPP, IPP1, PP1, PPase, SID6-8061

Gene Symbol

PPA1

Additional Inorganic Pyrophosphatase/PPA1 Products

Product Documents for Inorganic Pyrophosphatase/PPA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Inorganic Pyrophosphatase/PPA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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