Insulin Antibody (2D11-H5 (same as INS05))

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15195

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D11-H5 (same as INS05)

Concentration

0.2 mg/ml

View all Insulin Antibodies »

Product Specifications

Immunogen

Purified pig insulin, conjugated to KLH (Uniprot: P01308)

Localization

Cytoplasmic

Specificity

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Marker

beta-Cell & Insulinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33146)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Insulin Antibody (2D11-H5 (same as INS05))

Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5 (same as INS05)) [NBP2-15195]

Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5 (same as INS05)) [NBP2-15195]

Immunohistochemistry-Paraffin: Insulin Antibody (2D11-H5) [NBP2-15195] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal

Applications for Insulin Antibody (2D11-H5 (same as INS05))

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Insulin

Insulin is a secreted peptide hormone produced by the pancreas that regulates carbohydrate and lipid metabolism. Insulin is released from the pancreatic beta cells of the islets of Langerhans in response to secretagogues. Following its release, insulin quickly acts to both inhibit hepatic glucose production and stimulate peripheral blood glucose utilization. During this process cells in the liver, muscle, and fat are triggered to take up glucose and store it as glycogen, resulting in a decrease of blood glucose levels. From a clinical standpoint, the glucose lowering effect of insulin is its most relevant property. Insulin dysregulation, including insulin resistance, is associated with a number of pathological conditions such as diabetes, insulinoma, metabolic syndrome and polycystic ovary syndrome. Insulin is used to treat some forms of diabetes. Although antibody or auto-antibody against insulin isn't normally found in the blood, antibody may be produced in certain conditions including following an allergic response to insulin treatment for diabetes: IgG, IgM, and IgE antibody have all been described. The presence of auto-antibody, identified by an insulin antibody test, may have clinical significance by contributing to or causing insulin dysregulation.

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

UniProt

P01308

Additional Insulin Products

Product Documents for Insulin Antibody (2D11-H5 (same as INS05))

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Insulin Antibody (2D11-H5 (same as INS05))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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