Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D3E7 (5B6/6)

Concentration

1.0 mg/ml

View all Insulin Antibodies »

Product Specifications

Immunogen

Recombinant human insulin

Reactivity Notes

Predicted cross-reactivities: Porcine, Rat, Mouse, Bovine

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Secreted

Specificity

NB100-64697 recognises both insulin and proinsulin, but does not react with free C-peptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Insulin Antibody (D3E7 (5B6/6))

Immunohistochemistry-Paraffin: Insulin Antibody (D3E7 (5B6/6)) [NB100-64697] Staining of paraffin embedded human pancreas.

Applications for Insulin Antibody (D3E7 (5B6/6))

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Insulin

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Entrez Gene IDs

3630 (Human); 24505 (Rat)

Gene Symbol

INS

UniProt

P01308

Additional Insulin Products

Product Documents for Insulin Antibody (D3E7 (5B6/6))

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Insulin Antibody (D3E7 (5B6/6))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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