Insulin R/CD220 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55539

Novus Biologicals, part of Bio-Techne
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NB110-55539
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Predicted:

Bovine (94%), Canine (94%), Mouse (94%), Primate (100%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Insulin R/CD220 Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from internal region of human Insulin R/CD220.

Epitope

Internal domain of human

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Bat (88%), Equine (88%), Porcine (88%), Marmoset (81%).

Specificity

Human Insulin R/CD220. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except JARID2 (69%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Insulin R/CD220 Antibody - BSA Free

Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody - BSA Free [NB110-55539]

Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody - BSA Free [NB110-55539]

Immunohistochemistry-Paraffin: Insulin R/CD220 Antibody - BSA Free [NB110-55539] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Applications for Insulin R/CD220 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
IHC: NB110-55539 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Insulin R/CD220

The insulin receptor is a heterotetrameric membrane glycoprotein with tyrosine-protein kinase activity, consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit possesses a single transmembrane domain, whereas the alpha subunit is completely extracellular. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Binding of insulin to the insulin receptor stimulates its association with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K) which leads to glucose uptake. Two transcript variants encoding different isoforms have been found for this gene produced by alternative splicing. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Long Name

Insulin Receptor

Alternate Names

CD220, INSR, InsulinR

Entrez Gene IDs

3643 (Human)

Gene Symbol

INSR

Additional Insulin R/CD220 Products

Product Documents for Insulin R/CD220 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Insulin R/CD220 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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